Hyperglycemia facilitates dengue virus infection and host immune alteration

Abstract Patients with diabetes mellitus are highly comorbid severe dengue diseases caused by the virus (DENV). In search of possible host factors involved in DENV infection under hyperglycemic stress, cells and murine treated high glucose (HG) increase viral protein expression virion release but have no effects on antiviral interferon responses as well early infectious stage. Following HG stimulation, a cellular replicon-based assay displays an increased translation while uptake inhibitor phloretin treatment blocks such effect. Treating causes increases gene translational factor poly(A)-binding (PABP) transporter-associated PI3K/AKT-regulated manner. PABP silence further significant decrease HG-increased production. addition, enhances formation PABP-eukaryotic initiation 4G complex, regulated protein-disulfide isomerase. Furthermore, streptozotocin-treated mice reveal mortality, brain expression, loads infection. This study demonstrates that stress facilitates strengthening PABP-mediated translation. work is supported grants from National Science Technology Council (NSTC109-2327-B-006-010 110-2320-B-038-064-MY3), Taipei, Taiwan.